Brillouin microscopy
نویسندگان
چکیده
منابع مشابه
Brillouin microscopy, what is it really measuring?
Brillouin microscopy is a new tool to characterize the stiffness of cells and tissues, however the underlying relationship between stiffness and Brillouin measurements remains unknown. As the stiffness of biological materials is influenced by hydration, we performed Brillouin microscopy on hydrogels whilst independently examining the role of stiffness and water content. Brillouin measurements w...
متن کاملAssessing corneal biomechanics with Brillouin spectro-microscopy.
A new Brillouin spectro-microscope was designed and built to investigate the mechanical properties of bovine and human corneas. This instrument integrates a single-stage virtually imaged phased array spectrometer with a novel adaptive-optics interferometric filter to achieve unprecedented rejection of the elastic background signal. As a result, highly-resolved, reproducible data from both thin ...
متن کاملIn vivo Brillouin optical microscopy of the human eye
We report the first Brillouin measurement of the human eye in vivo. We constructed a Brillouin optical scanner safe for human use by employing continuous-wave laser light at 780 nm at a low power of 0.7 mW. With a single scan along the optic axis of the eye, the axial profile of Brillouin frequency shift was obtained with a pixel acquisition time of 0.4 s and axial resolution of about 60 μm, sh...
متن کاملLine-scanning Brillouin microscopy for rapid non-invasive mechanical imaging
Brillouin spectroscopy probes the mechanical properties of material by measuring the optical frequency shift induced by photon-phonon scattering interactions. In traditional configurations, Brillouin spectrometers measure only one point of the sample at a time. This results in long acquisition times for mechanical imaging of large areas. In this work, we demonstrate a parallel detection configu...
متن کاملBiomechanical characterization of keratoconus corneas ex vivo with Brillouin microscopy.
PURPOSE Loss of corneal strength is a central feature of keratoconus progression. However, it is currently difficult to measure corneal mechanical changes noninvasively. The objective of this study is to evaluate if Brillouin optical microscopy can differentiate the mechanical properties of keratoconic corneas versus healthy corneas ex vivo. METHODS We obtained eight tissue samples from healt...
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ژورنال
عنوان ژورنال: Current Opinion in Ophthalmology
سال: 2018
ISSN: 1040-8738
DOI: 10.1097/icu.0000000000000489